Details of experiments between Q8TAA9 and Q14160
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: VANGL1
Protein A fragment: 515-524
Protein A site: PBM
Protein A sequence: VLRLQSETSV
Protein A construct: biotin-ttds-VLRLQSETSV
Protein B protein: SCRIB
Protein B fragment: 718-814
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.79
Normalized holdup BI: 0.83
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.53
Experiment series 2
Protein A protein: VANGL1
Protein A fragment: 515-524
Protein A site: PBM
Protein A sequence: VLRLQSETSV
Protein A construct: biotin-ttds-VLRLQSETSV
Protein B protein: SCRIB
Protein B fragment: 858-949
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.37
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.46
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.72
Experiment series 3
Protein A protein: VANGL1
Protein A fragment: 515-524
Protein A site: PBM
Protein A sequence: VLRLQSETSV
Protein A construct: biotin-ttds-VLRLQSETSV
Protein B protein: SCRIB
Protein B fragment: 999-1093
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.33
Normalized holdup BI: 0.36
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.54