Details of experiments between Q8NI35 and O15439
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: Inadl
Protein A fragment: 1058-1167
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.25
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.58
Experiment series 2
Protein A protein: Inadl
Protein A fragment: 1530-1615
Protein A site: PDZ_9
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.33
Experiment series 3
Protein A protein: Inadl
Protein A fragment: 359-469
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.15
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.27
Experiment series 4
Protein A protein: Inadl
Protein A fragment: 673-780
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.05
Experiment series 5
Protein A protein: Inadl
Protein A fragment: 113-229
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: Inadl
Protein A fragment: 1673-1763
Protein A site: PDZ_10
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: Inadl
Protein A fragment: 238-335
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: Inadl
Protein A fragment: 547-651
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 9
Protein A protein: Inadl
Protein A fragment: 1219-1332
Protein A site: PDZ_7
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: -0.18
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: Inadl
Protein A fragment: 1425-1530
Protein A site: PDZ_8
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: Inadl
Protein A fragment: 1058-1167
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.5
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.55
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 4
Measured pKd: 4.89
Experiment series 12
Protein A protein: Inadl
Protein A fragment: 359-469
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.15
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.15
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4
Experiment series 13
Protein A protein: Inadl
Protein A fragment: 113-229
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.06
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0.06
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.53
Experiment series 14
Protein A protein: Inadl
Protein A fragment: 1673-1763
Protein A site: PDZ_10
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.06
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.06
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.52
Experiment series 15
Protein A protein: Inadl
Protein A fragment: 238-335
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: -0.04
Holdup BI standard deviation: 0.06
Normalized holdup BI: -0.04
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 16
Protein A protein: Inadl
Protein A fragment: 547-651
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: -0.04
Holdup BI standard deviation: 0.02
Normalized holdup BI: -0.04
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 17
Protein A protein: Inadl
Protein A fragment: 673-780
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.02
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.02
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 18
Protein A protein: Inadl
Protein A fragment: 1425-1530
Protein A site: PDZ_8
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 19
Protein A protein: Inadl
Protein A fragment: 1530-1615
Protein A site: PDZ_9
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Normalized holdup BI: -0.01
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 20
Protein A protein: InaDl
Protein A fragment: 113-229
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.08
Normalized holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 3.71
Experiment series 21
Protein A protein: InaDl
Protein A fragment: 1673-1763
Protein A site: PDZ_10
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 3.91
Experiment series 22
Protein A protein: InaDl
Protein A fragment: 238-335
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 3.63
Experiment series 23
Protein A protein: InaDl
Protein A fragment: 359-469
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.23
Normalized holdup BI: 0.27
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 4.34
Experiment series 24
Protein A protein: InaDl
Protein A fragment: 547-651
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 25
Protein A protein: InaDl
Protein A fragment: 673-780
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 26
Protein A protein: InaDl
Protein A fragment: 1058-1167
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.52
Normalized holdup BI: 0.57
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 4.93
Experiment series 27
Protein A protein: InaDl
Protein A fragment: 1425-1530
Protein A site: PDZ_8
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 3.48
Experiment series 28
Protein A protein: InaDl
Protein A fragment: 1530-1615
Protein A site: PDZ_9
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 29
Protein A protein: InaDl
Protein A fragment: 1425-1530
Protein A site: PDZ_8
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: Biotin-ado-ado-STLTIFETAL
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 30
Protein A protein: InaDl
Protein A fragment: 673-780
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: Biotin-ado-ado-STLTIFETAL
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 31
Protein A protein: InaDl
Protein A fragment: 359-469
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: Biotin-ado-ado-STLTIFETAL
Average holdup BI: 0.3
Normalized holdup BI: 0.3
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.4
Experiment series 32
Protein A protein: InaDl
Protein A fragment: 1673-1763
Protein A site: PDZ_10
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: Biotin-ado-ado-STLTIFETAL
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.6
Experiment series 33
Protein A protein: InaDl
Protein A fragment: 1530-1615
Protein A site: PDZ_9
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: Biotin-ado-ado-STLTIFETAL
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 34
Protein A protein: InaDl
Protein A fragment: 1058-1167
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: Biotin-ado-ado-STLTIFETAL
Average holdup BI: 0.6
Normalized holdup BI: 0.6
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.99
Experiment series 35
Protein A protein: InaDl
Protein A fragment: 547-651
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: Biotin-ado-ado-STLTIFETAL
Average holdup BI: -0.08
Normalized holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 36
Protein A protein: InaDl
Protein A fragment: 238-335
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: Biotin-ado-ado-STLTIFETAL
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 37
Protein A protein: InaDl
Protein A fragment: 113-229
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCC4
Protein B fragment: 1316-1325
Protein B site: PBM
Protein B sequence: STLTIFETAL
Protein B construct: Biotin-ado-ado-STLTIFETAL
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.