Details of experiments between the fragments Q8N743~401-410 and Q9C0E4~238-339
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: KIR3DL3
Protein A fragment: 401-410
Protein A site: PBM
Protein A sequence: PKTPPTDTSV
Protein A construct: biotin-ttds-PKTPPTDTSV
Protein B protein: GRIP2
Protein B fragment: 238-339
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: KIR3DL3
Protein A fragment: 401-410
Protein A site: PBM
Protein A sequence: PKTPPTDTSV
Protein A construct: biotin-ttds-PKTPPTDTSV
Protein B protein: GRIP2
Protein B fragment: 238-339
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.05
Normalized holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.