Details of experiments between the fragments Q8N743~401-410 and O15018~575-684
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: KIR3DL3
Protein A fragment: 401-410
Protein A site: PBM
Protein A sequence: PKTPPTDTSV
Protein A construct: biotin-ttds-PKTPPTDTSV
Protein B protein: PDZD2
Protein B fragment: 575-684
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.08