Details of experiments between Q8N743 and Q96QZ7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: KIR3DL3
Protein A fragment: 401-410
Protein A site: PBM
Protein A sequence: PKTPPTDTSV
Protein A construct: biotin-ttds-PKTPPTDTSV
Protein B protein: MAGI1
Protein B fragment: 1146-1238
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.22
Experiment series 2
Protein A protein: KIR3DL3
Protein A fragment: 401-410
Protein A site: PBM
Protein A sequence: PKTPPTDTSV
Protein A construct: biotin-ttds-PKTPPTDTSV
Protein B protein: MAGI1
Protein B fragment: 1-106
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: KIR3DL3
Protein A fragment: 401-410
Protein A site: PBM
Protein A sequence: PKTPPTDTSV
Protein A construct: biotin-ttds-PKTPPTDTSV
Protein B protein: MAGI1
Protein B fragment: 456-580
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: KIR3DL3
Protein A fragment: 401-410
Protein A site: PBM
Protein A sequence: PKTPPTDTSV
Protein A construct: biotin-ttds-PKTPPTDTSV
Protein B protein: MAGI1
Protein B fragment: 638-725
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: KIR3DL3
Protein A fragment: 401-410
Protein A site: PBM
Protein A sequence: PKTPPTDTSV
Protein A construct: biotin-ttds-PKTPPTDTSV
Protein B protein: MAGI1
Protein B fragment: 836-926
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: KIR3DL3
Protein A fragment: 401-410
Protein A site: PBM
Protein A sequence: PKTPPTDTSV
Protein A construct: biotin-ttds-PKTPPTDTSV
Protein B protein: MAGI1
Protein B fragment: 986-1098
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: KIR3DL3
Protein A fragment: 401-410
Protein A site: PBM
Protein A sequence: PKTPPTDTSV
Protein A construct: biotin-ttds-PKTPPTDTSV
Protein B protein: MAGI1
Protein B fragment: 1-106
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Normalized holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: KIR3DL3
Protein A fragment: 401-410
Protein A site: PBM
Protein A sequence: PKTPPTDTSV
Protein A construct: biotin-ttds-PKTPPTDTSV
Protein B protein: MAGI1
Protein B fragment: 456-580
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.08
Normalized holdup BI: -0.09
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.