Details of experiments between the fragments Q8N448~592-688 and Q9NYB5~703-712
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: LNX2
Protein A fragment: 592-688
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLCO1C1
Protein B fragment: 703-712
Protein B site: PBM
Protein B sequence: NYWPGKETQL
Protein B construct: biotin-ttds-NYWPGKETQL
Average holdup BI: 0.2
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.73