Details of experiments between Q8N448 and Q6ZTQ3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: LNX2
Protein A fragment: 334-426
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RASSF6
Protein B fragment: 360-369
Protein B site: PBM
Protein B sequence: KLVIKTETTV
Protein B construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: LNX2
Protein A fragment: 459-560
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RASSF6
Protein B fragment: 360-369
Protein B site: PBM
Protein B sequence: KLVIKTETTV
Protein B construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: LNX2
Protein A fragment: 592-688
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RASSF6
Protein B fragment: 360-369
Protein B site: PBM
Protein B sequence: KLVIKTETTV
Protein B construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: LNX2
Protein A fragment: 334-426
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RASSF6
Protein B fragment: 360-369
Protein B site: PBM
Protein B sequence: KLVIKTETTV
Protein B construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.33
Normalized holdup BI: 0.37
Immobilized partner concentration in holdup experiment (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.48
Experiment series 5
Protein A protein: LNX2
Protein A fragment: 459-560
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RASSF6
Protein B fragment: 360-369
Protein B site: PBM
Protein B sequence: KLVIKTETTV
Protein B construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.