Details of experiments between the fragments Q8N1I0~1957-1966 and Q14160~999-1093
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DOCK4
Protein A fragment: 1957-1966
Protein A site: PBM
Protein A sequence: RPLPRKVSQL
Protein A construct: biotin-ttds-RPLPRKVSQL
Protein B protein: SCRIB
Protein B fragment: 999-1093
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.33
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.76
Experiment series 2
Protein A protein: DOCK4
Protein A fragment: 1957-1966
Protein A site: PBM
Protein A sequence: RPLPRKVSQL
Protein A construct: biotin-ttds-RPLPRKVSQL
Protein B protein: SCRIB
Protein B fragment: 999-1093
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.41
Normalized holdup BI: 0.46
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.72