Details of experiments between the fragments Q8N103~722-731 and Q9Y3R0~464-567
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TAGAP
Protein A fragment: 722-731
Protein A site: PBM
Protein A sequence: QFQYAKESYI
Protein A construct: biotin-ttds-QFQYAKESYI
Protein B protein: GRIP1
Protein B fragment: 464-567
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.