Details of experiments between the fragments Q8N103~722-731 and P26045~505-597
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TAGAP
Protein A fragment: 722-731
Protein A site: PBM
Protein A sequence: QFQYAKESYI
Protein A construct: biotin-ttds-QFQYAKESYI
Protein B protein: PTPN3
Protein B fragment: 505-597
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.53
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.53
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.84