Details of experiments between Q8N103 and Q68DX3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TAGAP
Protein A fragment: 722-731
Protein A site: PBM
Protein A sequence: QFQYAKESYI
Protein A construct: biotin-ttds-QFQYAKESYI
Protein B protein: FRMPD2
Protein B fragment: 944-1038
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.24
Normalized holdup BI: 0.27
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.33
Experiment series 2
Protein A protein: TAGAP
Protein A fragment: 722-731
Protein A site: PBM
Protein A sequence: QFQYAKESYI
Protein A construct: biotin-ttds-QFQYAKESYI
Protein B protein: FRMPD2
Protein B fragment: 770-861
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: TAGAP
Protein A fragment: 722-731
Protein A site: PBM
Protein A sequence: QFQYAKESYI
Protein A construct: biotin-ttds-QFQYAKESYI
Protein B protein: FRMPD2
Protein B fragment: 1073-1166
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.