Details of experiments between the fragments Q8IZ21~286-300 and P00519~56-121
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PHACTR4
Protein A fragment: 286-300
Protein A site: PxxP_4
Protein A sequence: LLSKPSPPLPPKRGI
Protein A construct: Bio Ado Ado-LLSKPSPPLPPKRGI-CONH2
Protein B protein: ABL1
Protein B fragment: 56-121
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 2
The affinity was below the detection threshold of the assay.