Details of experiments between the fragments Q8IYB3~576-590 and P55345~24-96
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SRRM1
Protein A fragment: 576-590
Protein A site: PxxP_7
Protein A sequence: PPRRRTPSPPPRRRS
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: PRMT2
Protein B fragment: 24-96
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.11
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 4
Measured pKd: 3.84
Experiment series 2
Protein A protein: SRRM1
Protein A fragment: 576-590
Protein A site: PxxP_7
Protein A sequence: PPRRRTPSPPPRRRS
Protein A construct: Bio E E Ado Ado-PPRRRTPSPPPRRRS-CONH2
Protein B protein: PRMT2
Protein B fragment: 24-96
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.