Details of experiments between the fragments Q8IWK6~1312-1321 and Q14CM0~67-160
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ADGRA3
Protein A fragment: 1312-1321
Protein A site: PBM
Protein A sequence: TGLWKHETTV
Protein A construct: biotin-ado-ado-TGLWKHETTV
Protein B protein: FRMPD4
Protein B fragment: 67-160
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.