Details of experiments between Q8IVF5 and Q8TBB1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TIAM2
Protein A fragment: 878-980
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: LNX1
Protein B fragment: 719-728
Protein B site: PBM
Protein B sequence: TIVSWPGTFL
Protein B construct: Biotin-Ahx-TIVSWPGTFL
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.