Details of experiments between the fragments Q8IUC4~506-606 and P60484~391-403
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: RHPN2
Protein A fragment: 506-606
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: PTEN
Protein B fragment: 391-403
Protein B site: PBM
Protein B sequence: EDQHTQITKV
Protein B construct: Biotin-ttds-PFDEDQHTQITKV

Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 17
Experiment method: CALIP_holdup
Details of affinity fitting: lysozyme internal standard
PUBMED ID of publication: 33382810
Number of measurements: 1
The affinity was below the detection threshold of the assay.