Details of experiments between the fragments Q86UT5~106-208 and O15169~333-347
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: NHERF4
Protein A fragment: 106-208
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: AXIN1
Protein B fragment: 333-347
Protein B site: Internal_PBM
Protein B construct: Biotin-GSGSGS-TLSLTDSSVDGIPPYR-CONH2
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.