Details of experiments between the fragments Q86UR5~584-692 and P06427~140-149
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: RIMS1
Protein A fragment: 584-692
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV33-E6
Protein B fragment: 140-149
Protein B site: PBM
Protein B sequence: SRSRRRETAL
Protein B construct: biotin-ttds-SRSRRRETAL
Average holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.