Details of experiments between the fragments Q86UR5~584-692 and A6NIM6~570-579
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: RIMS1
Protein A fragment: 584-692
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: -0.19
Immobilized partner concentration in holdup experiment (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.