Details of experiments between the fragments Q86UL8~410-522 and Q6UVM3~1126-1135
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAGI2
Protein A fragment: 410-522
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KCNT2
Protein B fragment: 1126-1135
Protein B site: PBM
Protein B sequence: GQDSREETQL
Protein B construct: biotin-ttds-GQDSREETQL
Measured dissociation constant (10-6M): 248.32
Standard deviation of dissociation constant (10-6M): 19.39
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 3.6
Experiment series 2
Protein A protein: MAGI2
Protein A fragment: 410-522
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KCNT2
Protein B fragment: 1126-1135
Protein B site: PBM
Protein B sequence: GQDSREETQL
Protein B construct: biotin-ttds-GQDSREETQL
Average holdup BI: -0.07
Normalized holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 13.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.