Details of experiments between the fragments Q86UL8~1141-1229 and P35222~772-781
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAGI2
Protein A fragment: 1141-1229
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: CTNNB1
Protein B fragment: 772-781
Protein B site: PBM
Protein B sequence: NQLAWFDTDL
Protein B construct: biotin-ttds-NQLAWFDTDL
Average holdup BI: 0.57
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.68
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 5.15