Details of experiments between Q86UL8 and P0DTC3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAGI2
Protein A fragment: 1-102
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-3a
Protein B fragment: 266-275
Protein B site: PBM
Protein B sequence: EPTTTTSVPL
Protein B construct: Biotin-(PEG)3-YDEPTTTTSVPL
Average holdup BI: -0.15
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: MAGI2
Protein A fragment: 410-522
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-3a
Protein B fragment: 266-275
Protein B site: PBM
Protein B sequence: EPTTTTSVPL
Protein B construct: Biotin-(PEG)3-YDEPTTTTSVPL
Average holdup BI: -0.12
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: MAGI2
Protein A fragment: 600-682
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-3a
Protein B fragment: 266-275
Protein B site: PBM
Protein B sequence: EPTTTTSVPL
Protein B construct: Biotin-(PEG)3-YDEPTTTTSVPL
Average holdup BI: -0.05
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: MAGI2
Protein A fragment: 772-865
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-3a
Protein B fragment: 266-275
Protein B site: PBM
Protein B sequence: EPTTTTSVPL
Protein B construct: Biotin-(PEG)3-YDEPTTTTSVPL
Average holdup BI: -0.07
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: MAGI2
Protein A fragment: 915-1017
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-3a
Protein B fragment: 266-275
Protein B site: PBM
Protein B sequence: EPTTTTSVPL
Protein B construct: Biotin-(PEG)3-YDEPTTTTSVPL
Average holdup BI: -0.04
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: MAGI2
Protein A fragment: 1141-1229
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-3a
Protein B fragment: 266-275
Protein B site: PBM
Protein B sequence: EPTTTTSVPL
Protein B construct: Biotin-(PEG)3-YDEPTTTTSVPL
Average holdup BI: -0.05
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.