Details of experiments between Q86UL8 and O95477
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAGI2
Protein A fragment: 600-682
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCA1
Protein B fragment: 2252-2261
Protein B site: PBM
Protein B sequence: QDEKVKESYV
Protein B construct: Biotin-Ahx-QDEKVKESYV
Average holdup BI: 0.53
Normalized holdup BI: 0.53
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.85
Experiment series 2
Protein A protein: MAGI2
Protein A fragment: 915-1017
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCA1
Protein B fragment: 2252-2261
Protein B site: PBM
Protein B sequence: QDEKVKESYV
Protein B construct: Biotin-Ahx-QDEKVKESYV
Average holdup BI: 0.39
Normalized holdup BI: 0.39
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.58
Experiment series 3
Protein A protein: MAGI2
Protein A fragment: 1141-1229
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCA1
Protein B fragment: 2252-2261
Protein B site: PBM
Protein B sequence: QDEKVKESYV
Protein B construct: Biotin-Ahx-QDEKVKESYV
Average holdup BI: 0.33
Normalized holdup BI: 0.36
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.53
Experiment series 4
Protein A protein: MAGI2
Protein A fragment: 410-522
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCA1
Protein B fragment: 2252-2261
Protein B site: PBM
Protein B sequence: QDEKVKESYV
Protein B construct: Biotin-Ahx-QDEKVKESYV
Average holdup BI: 0.3
Holdup BI standard deviation: 0
Normalized holdup BI: 0.35
Normalized holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.52
Experiment series 5
Protein A protein: MAGI2
Protein A fragment: 772-865
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCA1
Protein B fragment: 2252-2261
Protein B site: PBM
Protein B sequence: QDEKVKESYV
Protein B construct: Biotin-Ahx-QDEKVKESYV
Average holdup BI: 0.13
Normalized holdup BI: 0.16
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.04
Experiment series 6
Protein A protein: MAGI2
Protein A fragment: 410-522
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCA1
Protein B fragment: 2252-2261
Protein B site: PBM
Protein B sequence: QDEKVKESYV
Protein B construct: Biotin-ado-ado-QDEKVKESYV
Average holdup BI: 0.43
Normalized holdup BI: 0.48
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.75
Experiment series 7
Protein A protein: MAGI2
Protein A fragment: 1141-1229
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCA1
Protein B fragment: 2252-2261
Protein B site: PBM
Protein B sequence: QDEKVKESYV
Protein B construct: Biotin-ado-ado-QDEKVKESYV
Average holdup BI: 0.43
Normalized holdup BI: 0.43
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.66
Experiment series 8
Protein A protein: MAGI2
Protein A fragment: 600-682
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCA1
Protein B fragment: 2252-2261
Protein B site: PBM
Protein B sequence: QDEKVKESYV
Protein B construct: Biotin-ado-ado-QDEKVKESYV
Average holdup BI: 0.47
Normalized holdup BI: 0.49
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.78
Experiment series 9
Protein A protein: MAGI2
Protein A fragment: 772-865
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCA1
Protein B fragment: 2252-2261
Protein B site: PBM
Protein B sequence: QDEKVKESYV
Protein B construct: Biotin-ado-ado-QDEKVKESYV
Average holdup BI: 0.09
Normalized holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.88
Experiment series 10
Protein A protein: MAGI2
Protein A fragment: 915-1017
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCA1
Protein B fragment: 2252-2261
Protein B site: PBM
Protein B sequence: QDEKVKESYV
Protein B construct: Biotin-ado-ado-QDEKVKESYV
Average holdup BI: 0.4
Normalized holdup BI: 0.4
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.6