Details of experiments between Q86UL8 and O43182
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAGI2
Protein A fragment: 915-1017
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARHGAP6
Protein B fragment: 965-974
Protein B site: PBM
Protein B sequence: NPDALPETLV
Protein B construct: biotin-ttds-NPDALPETLV
Average holdup BI: 0.33
Normalized holdup BI: 0.33
Immobilized partner concentration in holdup experiment (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.45
Experiment series 2
Protein A protein: MAGI2
Protein A fragment: 772-865
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARHGAP6
Protein B fragment: 965-974
Protein B site: PBM
Protein B sequence: NPDALPETLV
Protein B construct: biotin-ttds-NPDALPETLV
Average holdup BI: 0.23
Normalized holdup BI: 0.32
Immobilized partner concentration in holdup experiment (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.42
Experiment series 3
Protein A protein: MAGI2
Protein A fragment: 1141-1229
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARHGAP6
Protein B fragment: 965-974
Protein B site: PBM
Protein B sequence: NPDALPETLV
Protein B construct: biotin-ttds-NPDALPETLV
Average holdup BI: 0.22
Normalized holdup BI: 0.27
Immobilized partner concentration in holdup experiment (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.31
Experiment series 4
Protein A protein: MAGI2
Protein A fragment: 410-522
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARHGAP6
Protein B fragment: 965-974
Protein B site: PBM
Protein B sequence: NPDALPETLV
Protein B construct: biotin-ttds-NPDALPETLV
Average holdup BI: 0.17
Normalized holdup BI: 0.19
Immobilized partner concentration in holdup experiment (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.1
Experiment series 5
Protein A protein: MAGI2
Protein A fragment: 600-682
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARHGAP6
Protein B fragment: 965-974
Protein B site: PBM
Protein B sequence: NPDALPETLV
Protein B construct: biotin-ttds-NPDALPETLV
Average holdup BI: 0.1
Normalized holdup BI: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.89