Details of experiments between Q82005 and Q96HC4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV73-E6
Protein A fragment: 139-148
Protein A site: PBM
Protein A sequence: SGSRPSATVV
Protein A construct: biotin-ttds-SGSRPSATVV
Protein B protein: PDLIM5
Protein B fragment: 1-92
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.