Details of experiments between Q82005 and P83110
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV73-E6
Protein A fragment: 139-148
Protein A site: PBM
Protein A sequence: SGSRPSATVV
Protein A construct: biotin-ttds-SGSRPSATVV
Protein B protein: HTRA3
Protein B fragment: 353-453
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: HPV73-E6
Protein A fragment: 139-148
Protein A site: PBM
Protein A sequence: SGSRPSATVV
Protein A construct: biotin-ttds-SGSRPSATVV
Protein B protein: HTRA3
Protein B fragment: 353-453
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Normalized holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.7