Details of experiments between Q82005 and P78352
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV73-E6
Protein A fragment: 139-148
Protein A site: PBM
Protein A sequence: SGSRPSATVV
Protein A construct: biotin-ttds-SGSRPSATVV
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: HPV73-E6
Protein A fragment: 139-148
Protein A site: PBM
Protein A sequence: SGSRPSATVV
Protein A construct: biotin-ttds-SGSRPSATVV
Protein B protein: DLG4
Protein B fragment: 155-249
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.31
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.72
Experiment series 3
Protein A protein: HPV73-E6
Protein A fragment: 139-148
Protein A site: PBM
Protein A sequence: SGSRPSATVV
Protein A construct: biotin-ttds-SGSRPSATVV
Protein B protein: DLG4
Protein B fragment: 62-152
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.19
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.42
Experiment series 4
Protein A protein: HPV73-E6
Protein A fragment: 139-148
Protein A site: PBM
Protein A sequence: SGSRPSATVV
Protein A construct: biotin-ttds-SGSRPSATVV
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.11
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.12
Experiment series 5
Protein A protein: HPV73-E6
Protein A fragment: 139-148
Protein A site: PBM
Protein A sequence: SGSRPSATVV
Protein A construct: biotin-ttds-SGSRPSATVV
Protein B protein: DLG4
Protein B fragment: 155-249
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Normalized holdup BI: 0.19
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.14
Experiment series 6
Protein A protein: HPV73-E6
Protein A fragment: 139-148
Protein A site: PBM
Protein A sequence: SGSRPSATVV
Protein A construct: biotin-ttds-SGSRPSATVV
Protein B protein: DLG4
Protein B fragment: 62-152
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Normalized holdup BI: 0.14
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.97
Experiment series 7
Protein A protein: HPV73-E6
Protein A fragment: 139-148
Protein A site: PBM
Protein A sequence: SGSRPSATVV
Protein A construct: biotin-ttds-SGSRPSATVV
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Holdup BI standard deviation: 0.02
Normalized holdup BI: -0.01
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.