Details of experiments between the fragments Q81964~151-160 and Q9BXL7~660-756
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: CARD11
Protein B fragment: 660-756
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.06
Holdup BI standard deviation: 0.03
Normalized holdup BI: -0.06
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.