Details of experiments between Q81964 and Q8NEN9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV

Protein B protein: PDZD8
Protein B fragment: 359-461
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.