Details of experiments between Q81964 and Q68DX3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: FRMPD2
Protein B fragment: 770-861
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: FRMPD2
Protein B fragment: 944-1038
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: FRMPD2
Protein B fragment: 944-1038
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.11
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.95
Experiment series 4
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: FRMPD2
Protein B fragment: 770-861
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Holdup BI standard deviation: 0.11
Normalized holdup BI: 0.01
Normalized holdup BI standard deviation: 0.11
Immobilized partner concentration in holdup experiment (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: FRMPD2
Protein B fragment: 1073-1166
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Holdup BI standard deviation: 0.03
Normalized holdup BI: -0.02
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.