Details of experiments between Q81964 and Q5TCQ9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: MAGI3
Protein B fragment: 398-503
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.89
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 6.37
Experiment series 2
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: MAGI3
Protein B fragment: 847-941
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.2
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.62
Experiment series 3
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: MAGI3
Protein B fragment: 1-114
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: MAGI3
Protein B fragment: 572-655
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: MAGI3
Protein B fragment: 716-815
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: MAGI3
Protein B fragment: 1014-1103
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: MAGI3
Protein B fragment: 398-503
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.83
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.92
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 6.07