Details of experiments between Q81964 and Q14160
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: SCRIB
Protein B fragment: 718-814
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 39.22
Standard deviation of dissociation constant (10-6M): 1.79
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 4.41
Experiment series 2
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: SCRIB
Protein B fragment: 718-814
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: SCRIB
Protein B fragment: 858-949
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: SCRIB
Protein B fragment: 999-1093
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.15
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: SCRIB
Protein B fragment: 1090-1197
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: SCRIB
Protein B fragment: 858-949
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.17
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.18
Experiment series 7
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: SCRIB
Protein B fragment: 999-1093
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Holdup BI standard deviation: 0
Normalized holdup BI: 0.09
Normalized holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.86
Experiment series 8
Protein A protein: HPV59-E6
Protein A fragment: 151-160
Protein A site: PBM
Protein A sequence: QRQARSETLV
Protein A construct: biotin-ttds-QRQARSETLV
Protein B protein: SCRIB
Protein B fragment: 718-814
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.08
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 8
Measured pKd: 3.82