Details of experiments between Q7Z6J2 and Q9Y566
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GRASP
Protein A fragment: 386-395
Protein A site: PBM
Protein A sequence: RSLEEEESQL
Protein A construct: biotin-ttds-RSLEEEESQL
Protein B protein: SHANK1
Protein B fragment: 655-761
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.6
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.29
Experiment series 2
Protein A protein: GRASP
Protein A fragment: 386-395
Protein A site: PBM
Protein A sequence: RSLEEEESQL
Protein A construct: biotin-ttds-RSLEEEESQL
Protein B protein: SHANK1
Protein B fragment: 655-761
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.66
Normalized holdup BI: 0.66
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.1