Details of experiments between Q7Z6J2 and Q92614
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: GRASP
Protein A fragment: 386-395
Protein A site: PBM
Protein A sequence: RSLEEEESQL
Protein A construct: biotin-ttds-RSLEEEESQL

Protein B protein: MYO18A
Protein B fragment: 217-309
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.


Experiment series 2

Protein A protein: GRASP
Protein A fragment: 96-193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: MYO18A
Protein B fragment: 2045-2054
Protein B site: PBM
Protein B sequence: TEAKLTETNA
Protein B construct: Biotin-Ahx-TEAKLTETNA

Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.