Details of experiments between the fragments Q7Z628~587-596 and Q9C0E4~137-245
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: NET1
Protein A fragment: 587-596
Protein A site: PBM
Protein A sequence: SGGKRKETLV
Protein A construct: biotin-ttds-SGGKRKETLV
Protein B protein: GRIP2
Protein B fragment: 137-245
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 17.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
The affinity was below the detection threshold of the assay.