Details of experiments between the fragments Q7Z628~587-596 and Q5TCQ9~847-941
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: NET1
Protein A fragment: 587-596
Protein A site: PBM
Protein A sequence: SGGKRKETLV
Protein A construct: biotin-ttds-SGGKRKETLV
Protein B protein: MAGI3
Protein B fragment: 847-941
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Immobilized partner concentration in holdup experiment (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.72