Details of experiments between the fragments Q7Z628~587-596 and O75970~1141-1246
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: NET1
Protein A fragment: 587-596
Protein A site: PBM
Protein A sequence: SGGKRKETLV
Protein A construct: biotin-ttds-SGGKRKETLV
Protein B protein: MPDZ
Protein B fragment: 1141-1246
Protein B site: PDZ_7
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.43
Immobilized partner concentration in holdup experiment (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.35