Details of experiments between the fragments Q76G19~117-226 and P0DTC4~66-75
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PDZD4
Protein A fragment: 117-226
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-CoV2-E
Protein B fragment: 66-75
Protein B site: PBM
Protein B sequence: NSSRVPDLLV
Protein B construct: biotin-NLNSSRVPDLLV
Average holdup BI: -0.04
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.