Details of experiments between Q70Z35 and P59637
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: PREX2
Protein A fragment: 581-676
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: SARS-Cov-E
Protein B fragment: 67-76
Protein B site: PBM
Protein B sequence: SSEGVPDLLV
Protein B construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Average holdup BI: -0.02
Holdup BI standard deviation: 0.11
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.


Experiment series 2

Protein A protein: PREX2
Protein A fragment: 665-775
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: SARS-Cov-E
Protein B fragment: 67-76
Protein B site: PBM
Protein B sequence: SSEGVPDLLV
Protein B construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Average holdup BI: -0.03
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.