Details of experiments between the fragments Q6ZTQ3~360-369 and Q9H5P4~858-953
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: RASSF6
Protein A fragment: 360-369
Protein A site: PBM
Protein A sequence: KLVIKTETTV
Protein A construct: biotin-ttds-KLVIKTETTV
Protein B protein: PDZD7
Protein B fragment: 858-953
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Immobilized partner concentration in holdup experiment (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.31
Experiment series 2
Protein A protein: RASSF6
Protein A fragment: 360-369
Protein A site: PBM
Protein A sequence: KLVIKTETTV
Protein A construct: biotin-ttds-KLVIKTETTV
Protein B protein: PDZD7
Protein B fragment: 858-953
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.26
Normalized holdup BI: 0.32
Immobilized partner concentration in holdup experiment (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.39