Details of experiments between Q6ZTQ3 and Q92796
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: RASSF6
Protein A fragment: 360-369
Protein A site: PBM
Protein A sequence: KLVIKTETTV
Protein A construct: biotin-ttds-KLVIKTETTV
Protein B protein: DLG3
Protein B fragment: 221-314
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.45
Immobilized partner concentration in holdup experiment (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.06
Experiment series 2
Protein A protein: RASSF6
Protein A fragment: 360-369
Protein A site: PBM
Protein A sequence: KLVIKTETTV
Protein A construct: biotin-ttds-KLVIKTETTV
Protein B protein: DLG3
Protein B fragment: 124-219
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.18
Immobilized partner concentration in holdup experiment (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.43
Experiment series 3
Protein A protein: RASSF6
Protein A fragment: 360-369
Protein A site: PBM
Protein A sequence: KLVIKTETTV
Protein A construct: biotin-ttds-KLVIKTETTV
Protein B protein: DLG3
Protein B fragment: 381-476
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.25
Experiment series 4
Protein A protein: RASSF6
Protein A fragment: 360-369
Protein A site: PBM
Protein A sequence: KLVIKTETTV
Protein A construct: biotin-ttds-KLVIKTETTV
Protein B protein: DLG3
Protein B fragment: 221-314
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.69
Normalized holdup BI: 0.86
Immobilized partner concentration in holdup experiment (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.53
Experiment series 5
Protein A protein: RASSF6
Protein A fragment: 360-369
Protein A site: PBM
Protein A sequence: KLVIKTETTV
Protein A construct: biotin-ttds-KLVIKTETTV
Protein B protein: DLG3
Protein B fragment: 124-219
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.38
Normalized holdup BI: 0.39
Immobilized partner concentration in holdup experiment (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.52
Experiment series 6
Protein A protein: RASSF6
Protein A fragment: 360-369
Protein A site: PBM
Protein A sequence: KLVIKTETTV
Protein A construct: biotin-ttds-KLVIKTETTV
Protein B protein: DLG3
Protein B fragment: 381-476
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.27
Normalized holdup BI: 0.34
Immobilized partner concentration in holdup experiment (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.43