Details of experiments between Q6ZMN7 and Q96QZ7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PDZRN4
Protein A fragment: 1027-1036
Protein A site: PBM
Protein A sequence: HNAFLSVTTV
Protein A construct: Biotin-Ahx-HNAFLSVTTV
Protein B protein: MAGI1
Protein B fragment: 456-580
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.61
Experiment series 2
Protein A protein: PDZRN4
Protein A fragment: 1027-1036
Protein A site: PBM
Protein A sequence: HNAFLSVTTV
Protein A construct: Biotin-Ahx-HNAFLSVTTV
Protein B protein: MAGI1
Protein B fragment: 1-106
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.