Details of experiments between the fragments Q6UXZ0~253-262 and P78352~306-403
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TMIGD1
Protein A fragment: 253-262
Protein A site: PBM
Protein A sequence: DKDPHSETAL
Protein A construct: biotin-ttds-DKDPHSETAL
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 178.57
Standard deviation of dissociation constant (10-6M): 10.91
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 3.75
Experiment series 2
Protein A protein: TMIGD1
Protein A fragment: 253-262
Protein A site: PBM
Protein A sequence: DKDPHSETAL
Protein A construct: biotin-ttds-DKDPHSETAL
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.02
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18.1
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.