Details of experiments between Q6UXZ0 and Q5T2W1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TMIGD1
Protein A fragment: 253-262
Protein A site: PBM
Protein A sequence: DKDPHSETAL
Protein A construct: biotin-ttds-DKDPHSETAL
Protein B protein: NHERF3
Protein B fragment: 2-107
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.75
Normalized holdup BI: 0.8
Immobilized partner concentration in holdup experiment (10-6M): 18.1
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.42
Experiment series 2
Protein A protein: TMIGD1
Protein A fragment: 253-262
Protein A site: PBM
Protein A sequence: DKDPHSETAL
Protein A construct: biotin-ttds-DKDPHSETAL
Protein B protein: NHERF3
Protein B fragment: 375-459
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18.1
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.52
Experiment series 3
Protein A protein: TMIGD1
Protein A fragment: 253-262
Protein A site: PBM
Protein A sequence: DKDPHSETAL
Protein A construct: biotin-ttds-DKDPHSETAL
Protein B protein: NHERF3
Protein B fragment: 238-329
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18.1
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.