Details of experiments between Q6UVM3 and Q13884
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: KCNT2
Protein A fragment: 1126-1135
Protein A site: PBM
Protein A sequence: GQDSREETQL
Protein A construct: biotin-ttds-GQDSREETQL
Protein B protein: SNTB1
Protein B fragment: 107-196
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.23
Normalized holdup BI: 0.27
Immobilized partner concentration in holdup experiment (10-6M): 13.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.49