Details of experiments between the fragments Q6P0Q8~1102-1193 and Q9IR59~142-151
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV

Measured dissociation constant (10-6M): 12.58
Standard deviation of dissociation constant (10-6M): 0.44
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit

PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 4.9

Experiment series 2

Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV

Average holdup BI: 0.42
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.94

Experiment series 3

Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV

Average holdup BI: 0.79
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.79
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 5.18