Details of experiments between the fragments Q6P0Q8~1102-1193 and Q92614~2045-2054
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: MYO18A
Protein B fragment: 2045-2054
Protein B site: PBM
Protein B sequence: TEAKLTETNA
Protein B construct: Biotin-Ahx-TEAKLTETNA
Average holdup BI: -0.05
Holdup BI standard deviation: 0.1
Normalized holdup BI: -0.05
Normalized holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.