Details of experiments between the fragments Q6P0Q8~1102-1193 and Q8N448~681-690
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: LNX2
Protein B fragment: 681-690
Protein B site: PBM
Protein B sequence: TVICWPGSLV
Protein B construct: Biotin-Ahx-TVICWPGSLV
Average holdup BI: 0.18
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.18
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.1