Details of experiments between the fragments Q6P0Q8~1102-1193 and O00592~549-558
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: PODXL
Protein B fragment: 549-558
Protein B site: PBM
Protein B sequence: DLDEEEDTHL
Protein B construct: biotin-ttds-DLDEEEDTHL
Average holdup BI: 0.75
Normalized holdup BI: 0.75
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.31