Details of experiments between Q6P0Q8 and Q8N743
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KIR3DL3
Protein B fragment: 401-410
Protein B site: PBM
Protein B sequence: PKTPPTDTSV
Protein B construct: biotin-ttds-PKTPPTDTSV
Average holdup BI: 0.21
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.47
Experiment series 2
Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KIR3DL3
Protein B fragment: 401-410
Protein B site: PBM
Protein B sequence: PKTPPTDTSV
Protein B construct: biotin-ttds-PKTPPTDTSV
Average holdup BI: 0.45
Normalized holdup BI: 0.45
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.7